2017 June 26


Laboratory of Analytics

NeuroProteomics Laboratory

Laboratory of AnalyticsThe laboratory is one of the best workshops in the field of separation sciences (electrophoresis, chromatography), mass spectrometry and proteomics in Hungary. Until now, we have analyzed over thousand new synthetic peptides by mass spectrometry, and identified several thousand proteins from wide variety of biological sources: virus, bacteria, cell culture, worm, lucern, soya, wheat, barley, triticale, mouse, rat, goose, turkey and human. Our over 30 years experiences in separation sciences, 15 years work in mass spectrometry and proteomics, and our state of the art instrumentation guarantee the qualitative and quantitative analysis of proteins from biological samples at high level. Our laboratory at the Department of Medical Chemistry (DMC) is the member of the Neurobiological Research Centre at Szeged. Main research profile of the laboratory is the development of qualitative and quantitative proteomic methods for the research of neurodegenerative diseases. We are involved in a project of drug design for treating and curing neurodegeneration and memory loss.


Key staff involved

Tamás Janáky, PhD, associate professor, has been working for 30 years on the analysis of biologically active compounds including amino acids, peptides, proteins and steroids. His current interest is the development and application of separation, qualitative and quantitative mass spectrometric and bioinformatic methods for analysis of different proteomes. His research focuses on the molecular underpinnings of brain diseases (anxiety, depression, Alzheimer’s disease, Huntington disease). Together with Prof. Penke he established the first laboratory for proteomics in Hungary. He is the president of the Hungarian Proteomics Society. He organized many courses on separation sciences and proteomics.

Zoltán Szabó, PhD is working at the DMC since 2005. He is responsible for running LC-MS system(s) and software packages applied in the field of quantitative and qualitative proteomics. He has experience in using and customizing of several search engines (eg. Mascot, X! Tandem, Phenyx, Proteinlynx Global Server, Spectrum Mill etc.), and building and maintaining protein sequence databases used by those software. He performs label free quantitative proteomics measurements using a Waters Protein Expression System and analysis with Proteinlynx Global Server complemented with different open source analysis and statistical software. He can use his programming experience in pipelining between different software systems, and in automatic data collection, filtering and report generation.

Zoltán Kele PhD is working at the DMC since 1995. He is responsible for running triple quadrupole mass spectrometers and their coupled analytical techniques.

Róbert Berkecz, analytical chemist, he acquired the using of instrumental analytical techniques such as CE, HPLC and MS (ion trap, Triple quadruple, FTICR) during Ph.D. years. The main topic of his Ph.D. work is chiral discrimination of amino acids in liquid and gas phase. Presently he has focused the qualitative and quantitative analytical HPLC-MS methods of peptides, proteins and phospholipids in the last half years, since he works at DMC.


Instrumentation

  Separation and detection of peptides, proteins

  • Preparative low-pressure chromatograph (Pharmacia, Sweden)
  • Series 1100 HPLC with UV and fluorescent detection (Agilent, Germany)
  • Series 1100 nanoHPLC (Agilent, Germany)
  • nanoAcquity nanoUPLC system (Waters)
  • PROTEAN IEF Isoelectric focusing system (BioRad)
  • SE900 electrophoretic unit for 28 cm x 21 cm PAGE gels (6)
  • FLA-5100 Fluorescent Scanner (FujiFilm)

  Mass spectrometers

  • QTOF Premier tandem mass spectrometer (Waters)
  • TSQ 7000 triple-quad mass spectrometers (Finnigan)
  • (both of the mass spectrometers are connected on-line to HPLC or nanoUPLC system)

  Bioinformatics softwares

  • Progenesis SameSpot for evaluation of 2D PAGE (Nonlinear Dynamics Ltd.)
  • MASCOT Server search engine (in-house server, ver. , Matrix Science Ltd.)
  • ProteinLynx Global Server ver. 2.4 (Waters Ltd.)
  • Scaffold protein identification software ver. 3.0 (Proteome Software Inc.)
  • Pathway Studio for pathway analysis ver. 7 (Ariadne Genomics Inc.)

Publications in Pubmed

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Webcam

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